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A comprehensive review on in-vitro methods for anti- microbial activity

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Review Article

Author Details : KM Krati*, Jaya Martolia, Neelam Painuly, Amandeep Singh

Volume : 9, Issue : 3, Year : 2024

Article Page : 157-165

https://doi.org/10.18231/j.ijcaap.2024.023

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Abstract

Microbes, also referred to as microscopic creatures because of their minuscule size and invisibility to human sight, are single-celled microorganisms. The first microorganism was discovered between 1665 and 1683 by Robert Hooke and Antoni van Leuwenhoek. There are two categories of techniques for examining microbial activity. methods used in vitro and in vivo. Experiments and tests carried out in a laboratory setting, typically without the presence of a living organism, are referred to as in vitro methods. By creating microbial cultures, cell lines, cell cultures, etc., we may do in vitro experiments. Scientists can study biological processes, assess the effects of medications, and understand systems without having to deal with the complexities of a whole organism by employing in vitro techniques. Growing cells outside of the body in a controlled setting for a range of studies is known as cell culture. Tissues are grown and kept in a lab setting for tissue culture. It is essential to research creating anti-infection therapies. To evaluate a substance's antibacterial activity, in vitro methods such as the brothdilution method and discdiffusion method are commonly used. There are different methods available for the testing of antimicrobial activity and the methods are the agar disk diffusion method, Agar well diffusion method, cross streak method, Bioautography, dilution method, Time kill test, ATP bioluminescence etc. Techniques for determining novel antimicrobial agents and testing for antibiotic susceptibility have been employed. Compared to in-vivo procedures, in vitro methods are less costly. To guarantee an accurate experimental approach, these techniques may be the answer.

Keywords: Micro­organism, Antimicrobial testing, Invitro method, Disk diffusion method, Time kill test 1

How to cite : Krati K, Martolia J, Painuly N, Singh A, A comprehensive review on in-vitro methods for anti- microbial activity. IP Int J Compr Adv Pharmacol 2024;9(3):157-165

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