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Tandam mass spectrometry instrumentation and application in pharmaceutical analysis

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Review Article

Author Details : Tarashankar Basuri*, Swatika S. Varli

Volume : 9, Issue : 2, Year : 2024

Article Page : 98-108

https://doi.org/10.18231/j.ijcaap.2024.015

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Abstract

Proteins and peptides can be analyzed using mass spectrometry (MS) using a range of techniques, including matrix-aided laser desorption ionization-mass spectrometry (MALDI-MS) and electrospray ionization-mass spectrometry (ESI-MS). These techniques make it possible to determine a protein's mass as an intact molecule or to identify a protein using peptide-mass fingerprinting that is produced during enzymatic digestion. The amino acid sequence of proteins (top-down and middle-down proteomics) and peptides (bottom-up proteomics) can be ascertained by fragmenting the proteins and peptides using tandem mass spectrometry (MS/MS). Furthermore, post-translational modifications (PTMs) of proteins and peptides can be identified using tandem mass spectrometry. In this article, we go over the use of MS/MS in biomedical research and provide concrete examples of how to identify proteins, peptides, and their PTMs as useful biomarkers for diagnosis and treatment. In numerous applications, tandem mass spectrometry (MS/MS) has shown to be a practical and efficient analytical method for the direct detection of target compounds in food samples. It combines the power of MS/MS as an identification and confirmation approach with the separation capabilities of chromatography when used with chromatographic techniques.

Keywords: Mass spectrometry: Ms­ms spectrometry, MS 2, Tandem mass spectrometry, Analytical method, Protein)

How to cite : Basuri T, Varli S S, Tandam mass spectrometry instrumentation and application in pharmaceutical analysis. IP Int J Compr Adv Pharmacol 2024;9(2):98-108

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