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Determination of amount of retinylpalmitate and ascorbic acid of extract gel of sweet corn fibre by UVand HPTLC method

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Original Article

Author Details : N Sunitha*, S Manohar Babu, B Appa Rao

Volume : 6, Issue : 4, Year : 2021

Article Page : 201-204

https://doi.org/10.18231/j.ijcaap.2021.036

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Abstract

Sweet corn fibres of about 15 gm were extracted with methanol for 5 hours in heating mantle at 40C and filtered and allowed to dry. The dried gel was further analyzed for estimation of Retinyl palmitate by spectrophotometrically by laboratory method and found to be 140 mg/kg. The dried extract gel was further estimated for ascorbic acid both by UV and HPTLC and found to be linear in the range of 1-5 ug/ml and 5-10ug/ml, correlation coefficient was found to be 0.997 and 0.998 and the amount of ascorbic acid was found to be 338 ng/ml and 9.9 ng/ml by UV and HPTLC respectively. The method was found to be linear.
 

Keywords: Sweet corn fibres, Retinyl Palmitate, Ascorbic acid, UV, HPTLC

How to cite : Sunitha N , Babu S M, Rao B A, Determination of amount of retinylpalmitate and ascorbic acid of extract gel of sweet corn fibre by UVand HPTLC method. IP Int J Compr Adv Pharmacol 2021;6(4):201-204

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