Study of hemp tribe HEMPOWHER granules on DHEA- induced PCOS in Sprague Dawley (SD) rat model

• Author Details:   
• Leela Priyanka G C
• Balasekar Premkumar ^*
• Arumugam Meena
• Arumugam Shanthy
• Manepalli Nageswara Rao Gupta
• Gopalakrishnan Saravanan
• Sallabuthula Rajkumar

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Background: Polycystic ovary syndrome (PCOS) is the common hormonal condition that affects adult women of reproductive age. PCOS can cause hormonal imbalance, excess androgen levels, and cysts in the ovary; irregular periods with a lack of ovulation can make it difficult to become pregnant. Lifestyle changes can improve PCOS symptoms, but medications and fertility treatments are not effective. The goal of this study is to see how Hemp Tribe HempowHer effects PCOS caused by Dehydroepiandrosterone (DHEA) in rats, and to compare it with the standard drug metformin hydrochloride.

Materials and Methods: DHEA was injected into a group of female rats to induce PCOS. Twenty-one days after induction of PCOS, Hem Tribe HempowHer granules and Metformin Hydrochloride were administered to the PCOS-induced animals orally for 14 days. Their body weight, hormone levels, estrous cycles, and histopathological studies were conducted and compared with the normal, standard metformin hydrochloride-administered animals.

Results: The results indicated body weight reduction in both the metformin hydrochloride-treated group and the HempowHer-treated group. Vaginal smear after 21 days of PCOS induction and 14 days after treatment with HempowHer showed normalization of the estrous cycle when compared to PCOS-induced groups. Hormonal analysis 21 days after induction of PCOS followed by 14 days of treatment with HempowHer significantly regulated the hormonal levels of luteinizing hormone, testosterone, progesterone, prolactin, and insulin when compared to the PCOS-induced group. Significant reduction of atretic follicles and increase in corpus luteum shown in histopathological studies of HempowHer-treated animals when compared to the PCOS-induced group indicate that the HempowHer supplement increases the possibility of the regulation of the estrous cycle, reduces the formation of ovary cysts, and improves ovary health.

Conclusion: The HempowHer, as per the study, regulates the estrous cycle and hormonal levels pertaining to ovary health and reproduction, reduces cyst formation, and ameliorates symptoms of PCOS.

Keywords: Cannabinoids, Women's health, Fertility, Ovary, Preclinical, Androgen.